Fractionation of Mouse malarious blood according to parasite developmental stage, using a percoll-sorbitol gradient
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Preparation of synaptoneurosomes from mouse cortex using a discontinuous percoll-sucrose density gradient.
Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that break away from axon terminals when the cortical tissue is homogenized. The SNs retain pre- and postsynaptic characteristics, which makes them useful in the study of synaptic transmission. They retain the molecular machinery used in neuronal si...
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Two methods of enucleation of LB 10 cells, a subline of mouse L cells, were used: the method of enucleation of cells growing in monolayers, and the newly improved method of enucleation in discontinuous Percoll gradients. The second method was more effective and, as shown by incorporation of 3H-lysine, protein synthesis in cytoplasts was prolonged twice when compared with that in cytoplasts obta...
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Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.
متن کاملDevelopmental Bias in Cleavage-Stage Mouse Blastomeres
BACKGROUND The cleavage-stage mouse embryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner cell mass (ICM) and the supportive trophectoderm (TE). However, it remains unsettled whether the contribution of each blastomere to these two lineages can be accounted for by chance. Addressing the question of blastomere cell fate may be of practical importa...
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ژورنال
عنوان ژورنال: Annales de Parasitologie Humaine et Comparée
سال: 1987
ISSN: 0003-4150
DOI: 10.1051/parasite/1987625418